We synthesized control full-length β-carotene hydroxylase mRNA using an RNA transcription kit (Stratagene). First strand cDNA synthesis was carried out using reverse primer 5 ...

Prolyl 3-hydroxylase 1 (P3H1 ... To identify alternatively spliced transcripts, RNA (1 μg) was reverse transcribed using antisense primers, e7as or e11as, specific for the LEPRE1 transcript ...