Gene knockout models involve the deliberate ... or nonhomologous end-joining (NHEJ). Historically, homologous recombination ...

One involves studying the effect of a 'candidate' gene on a phenotype; for example, using transgenesis or homologous recombination (creating 'knockout' or 'knockin' mice). The other entails using ...

It is thought that these proteins play a role in preventing recombination during DNA replication when it is not needed. An alternate hypothesis proposes that homologous chromosomes reside in pairs ...

To illuminate these issues, our lab has developed and refined methods for precisely mutating genes in human somatic cells via homologous recombination ... (i.e., conditional or constitutive “knockout” ...

Homologous recombination plays essential roles in the repair of DNA double strand breaks (DSBs) and collapsed replication forks. The fundamental reaction in homologous recombination is the exchange of ...

The investigation involved a comprehensive approach, including the generation of a Setd2-CD knockin mouse model through homologous recombination ... genes in both Setd2-CD and Setd2 knockout ...