What’s so special about Horizon’s HAP1 cell line? Because these cells, isolated from a leukemia patient and made to grow rapidly on lab plates, are near-haploid, ...

Hap1 Cells stained for different DNA damages (yellow = antibody against phosphorilated ATM; red = antibody against 53BP1; a regulator of NHEJ; blue = DAPI = DNA).

This is immunofluorescence staining of HAP1 cells lacking FANCC gene, treated for 24h with MMC, stained for BRCA1, 53BP1 and DAPI.

Cell fitness was used as a biological readout for BAP1 function, ... The refined SGE procedure improved transfection efficiency among HAP1 cells to above 60% from below 5.0%.