Engineering the replication of target DNA through cloning, or changing its genetic code through mutations, are detail-oriented processes whose foibles can spell disaster. Caitlin Smith looks at ...

These include transcription initiation of mRNA encoding genes, mRNA splicing, and mRNA polyadenylation ... biochemical fractionation and protein purification, cDNA and genomic DNA cloning, production ...

To generate the hairpin primer, select a 'sense' sequence(s) of 22 nucleotides (nt) in length from the coding sequence of the gene of interest ... miR-30 shRNA cloning experiments with reasonable ...

We are engaging proteins both as tools and as targets in the development of new technologies. Our methodology includes gene cloning for protein expression, mutagenesis, protein splicing, enzymology, ...