Thus, fused polypeptides may help producing GFP variants with a more robust folding, e.g. when poorly folding spectral variants are used or when fusion to insoluble proteins is needed. The GFP ...

Their improved-folding variant contained six new mutations, and they termed it superfolder GFP. As expected, the color variants of GFP could also be made from superfolder GFP, but the method is ...

The GFP reporting system was then used to test the specificity of these variants. The variant ChCas12b-D496A showed less off-target activity according to GUIDE-seq analysis, which might make it a ...