joining the GFP sequence at the amino- or carboxy-terminal end of the target protein as appropriate. If desired, subclone the DNA fragment encoding the GFP fusion protein into the pIRES vector.

Figure 1: GFP-fragment reassembly vectors and cloning linker sequences. To prepare the cloning vectors, transform separately pET11a-link-NGFP and pMRBAD-link-CGFP into an E. coli strain that gives ...

They began by training a type of model known as a convolutional neural network (CNN) on experimental data consisting of GFP ...