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as well as flow cytometers and microplate-based cytometers. Its far-red emission makes the stain ideal for multiplexing with common fluorescent probes, such as GFP.
“Flow cytometry has been around for many years ... transfected genes carry some kind of fluorescent tag, such as a GFP molecule, the expressed protein is as close to the native configuration ...
Flow cytometry is a widely used analytical technique that distinguishes between cell populations depending on the presence or absence of chosen markers. However, the number of markers that can be ...
GFP, mRFP, mCherry, tdTomato, mKate, mPlum). Applications that may benefit from access to this equipment includes: Construction and analysis of designed gene regulatory networks in bacteria.
GFP can be analysed with YFP on flow cytometers with appropriate filter and mirror changes. The GFP detector on a BD instrument should have a 502LP mirror with a 510/10nm BP filter. While the PE ...
the original CFSE being in the same channel as GFP. If violet laser diodes are not available, a Red HeNe diode can be used to measure cell proliferation by the use of Cell Trace Far Red DDAO-SE. Cell ...
This has advanced the use of flow cytometry in an increasing number of ... special applications such as side population assay, basic GFP sorting or optimized for multiple fluorescent protein ...
The University's flow cytometry facility is a core facility based in the Royal Hallamshire Hospital and provides a service to all at the University and externally. Flow cytometry is a technique that ...
The intuitive software can be mastered in minutes and presentations can be made by a drag and drop of plots into Microsoft Office applications. The BDFACSCelesta Flow Cytometer multicolor analyzer ...
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