Scientists have spent decades genetically modifying the bacterium Escherichia coli and other microbes to convert carbon dioxide into useful biological products. Most methods require additional carbon ...

Taking a risk on error-prone PCR, Euskirchen amplified only the GFP coding sequence and inserted it into an expression vector to transform back into E. coli. When Euskirchen imaged her bacteria using ...

The gene coding for GFP comes from the bioluminescent jellyfish ... Beta-lactamase is an enzyme that will allow transformed bacteria to be resistant to antibiotics that have a beta-lactam ring. Thus, ...

The GFP fluorescence (F) was quantified by fluorescence spectroscopy of whole-cell lysates. wtGFP expressed in bacteria was purified to homogeneity and used as a standard in western blotting and ...

1) Figure 1: GFP-fragment reassembly vectors and cloning ... Grow the cultures at 37 °C overnight in a shaking incubator Alternatively, bacteria from an agar plate may be recovered using a ...

These proteins, produced by some bacteria and algae ... the researchers set out to overcome those challenges, using data from GFP to develop and test a computational model that could predict ...

Caption Primary HUVECS expressing GFP targeted to the mitochondria. One of the HUVEC is infected with Chlamydia, stained against HSP60 (magenta).These cells were treated with 1 um H2O2 which ...