The GFP fluorescence (F) was quantified by fluorescence spectroscopy of whole-cell lysates. wtGFP expressed in bacteria was purified to homogeneity and used as a standard in western blotting and ...

Figure 1: GFP-fragment reassembly vectors and cloning ... Grow the cultures at 37 °C overnight in a shaking incubator Alternatively, bacteria from an agar plate may be recovered using a plate ...

This newly acquired DNA may include genes that enable bacteria to perform tasks that were previously impossible. For example, a gene that enables the microorganism to digest oil spills or perhaps more ...