Store the fractions at 4 °C for subsequent RP-HPLC or SDS-PAGE or at −20 °C ... the dead volume of the plumbing from the detector flow cell to the outlet tubing must be determined accurately, ...

The UV detector flow cell is smaller; less than one microliter, compared with 8-10 microliters in HPLC. A smaller flow cell controls dispersion or band broadening so that narrow peaks are maintained.

By improving the vacuum degasser flow channel design, users are able to achieve higher efficiency degassing, in a more streamlined device that sustains the use of crucial materials such as Teflon™ AF.

AB: Which UHPLC features will the ACQUITY® ArcTM system add that distinguishes it from HPLC systems? EG: The most differentiated feature of the ACQUITY Arc UHPLC System is the Arc Multi-flow path ...