using a fluorochrome that is still relatively bright but does not overlap with FITC. This example illustrates two additional rules: reserve the brightest fluorochromes for dim antibodies and vice ...

The optimal dilutions for anti-desmin and anti-αB-crystallin antibodies were 1/100 and 1/ ... anti-mouse immunoglobulin (Amersham) and a FITC-labelled anti-rabbit immunoglobulin (Tago ...