In the third step, the luciferin derivative is used to produce a bioluminescence signal with firefly luciferase. The wavelength can be used to differentiate between different OPs.

Firefly luciferase has been harnessed for biological research by coupling the luciferase reaction with ... at 677 nm. This wavelength can penetrate the bodies and tissues of most animals ...

While the emission wavelength of bioluminescence produced by AkaLumine yielded high ... AkaLumine-HCl emitted near-infrared bioluminescence at 677 nm when reacted with firefly luciferase, ...

BMG Labtech’s POLARStar Omega multi-mode spectrophotometer was used to take readings at 510nm wavelength using 5µl of a solution containing Firefly luciferase and luciferin introduced into the ...

While the emission wavelength of bioluminescence produced by AkaLumine yielded high ... AkaLumine-HCl emitted near-infrared bioluminescence at 677 nm when reacted with firefly luciferase ...