Reporter gene expression. Plasmid DNA (35 μg in 25 μl PBS) encoding firefly luciferase (pCMV-Luc; Aldevron, Fargo, North Dakota) was administered as above. Then, 24 h after delivery, full ...

He extracted two components from beetle abdomens, naming the substrate luciferine and the enzyme responsible for the reaction luciferase. 2 Not all bioluminescent marine organism systems can be used ...

These luciferase expressing cell lines were derived from parental lines by transduction with lentiviral vector encoding firefly luciferase gene (luc2) under control of EF-1 alpha promoter. These cell ...

Inspired by these elegant natural mechanisms, the research team developed a reporter molecule based on firefly luciferase (FLuc) that operates in a similar manner. Luciferase is a bioluminescence ...