Three general approaches have been used to develop comparable FT4 and FT3 immunoassay methods: (i) two-step labeled-hormone; (ii) one-step labeled-analog; and (iii) labeled antibody. Guideline 13.

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"A higher FT3/FT4 ratio was associated with an increased risk of developing NAFLD (OR 1.45) and greater severity of liver fibrosis (ORs 3.21–10.80), highlighting the potential role of thyroid hormones ...