Bacmids with high protein yields were selected for large-scale expression. An N-terminal superfolder GFP tag was introduced into the BacMam α7-nAChR vector. HEK293F cells were grown in routine ...

This construct was packaged into a replication defective adenoviral vector (Ad/5HRE-3NRSE) to determine neuron-selective and hypoxia-inducible gene expression in cultured mouse postmitotic primary ...

The Baculovirus Expression Vector System (BEVS) is used to produce recombinant proteins. This is very useful in regards to virology studies and biotechnology applications. Baculovirus on ice ready ...

The steps to create a stable, manufacturing cell line include host cell line selection, engineering of the expression vector, transfection, cloning and cell expansion, as well as various screening ...

Retroviral vectors that are both non-replicating and non-integrating represent a novel and attractive tool that has potential advantages for gene delivery therapy, immunotherapy, and vaccinology.

This novel vector technology with its persistent payload expression offers certain advantages over other gene delivery systems (DNA, mRNA, viral) for transient protein expression and use in gene ...

In biopharmaceutical manufacturing, the development of an expression system that stably ... In brief, this process requires a vector containing the geneselect s) of interest to be transfected ...

Data highlights advantages of single, non-replicative HSV-1 (nrHSV-1) vector hosting two transgenes with independent expression kineticsVectors also show high transduction rate of neurons in the CNS ...

An increase in serum choline levels to predict progression-free survival (PFS) in patients (pts) with advanced cancers receiving pembrolizumab. A phase I, dose-escalation study of ADG106, a fully ...

Our vector technology uniquely enables the delivered RNA payload to be persistently expressed for days without integrating into the cellular genomes. This improves upon the persistence of nonviral ...