The cell cycle has been the subject of intense investigation during two periods since Virchow's 1855 realization that cells only arise from pre-existing cells. At the turn of the century ...

The Flow Cytometry and Cell Separation Facility provides advanced cell sorting and cell analysis techniques including immunofluorescence detection of 18 colors, cell-cycle distribution, apoptosis ...

Harvest cells, want good single cell suspension Count cells Dispense aliquots of 1 million cells into labeled tubes Add 5 ml PBS and centrifuge for 5 min at 400 x g. Re-suspend cell pellet in 300 ul ...

Two independent groups, led by Steven Gygi of Harvard University Medical School and ... large-scale phosphoproteomic analyses across the cell cycle (Dephoure et al., 2008; Daub et al., ...

Previous studies revealed a positive correlation between O-GlcNAcylation and tumor growth via the stabilization of target proteins, with O-GlcNAcylation transferase (OGT) being the only enzyme capable ...