One-step creation of Cre-loxP organisms: Precise control and modification of gene expression
Researchers at the University of Tsukuba have developed a vector that integrates the Cre enzyme gene with the specific DNA ...
Nature1 年
Detecting protein-protein interactions with GFP-fragment reassembly
To prepare the cloning vectors, transform separately pET11a-link-NGFP and pMRBAD-link-CGFP into an E. coli strain that gives ... Purify the vector products by electrophoresis through 0.8–1% ...
Nature1 年
The Overnight Express Autoinduction System: High-density cell growth and protein expression ...
The Overnight Express Autoinduction Systems enable regulated protein expression in Escherichia coli without the need to monitor the culture or add inducer during cell growth and are marketed ...