PCR 即聚合酶链反应,是一种核酸扩增技术,能在体外快速复制 DNA 或 RNA 片段,就像 “分子影印”。1983 年,美国生物化学家 Kary Mullis 突发灵感,后来这项技术让他在 1993 年获诺贝尔化学奖 。
With the ever-growing popularity of quantitative PCR (QPCR) as a sensitive technique for quantifying DNA and RNA, there is increasing demand for higher throughput of reactions and faster protocol ...
精确扩增对于采用SYBR Green I技术的RT-qPCR的成功至关重要,因为这种染料会结合扩增过程中产生的所有双链DNA。这种一步法试剂含有超纯的AccuStart热 ...
qPCR, also known as real-time PCR, is an extension of traditional PCR, where DNA amplification over time is quantitated by measuring fluorescence from dyes intercalating into nascent DNA strands or by ...
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(EvaGreen™ 完整的安全性报告可以从Biotium网站上下载) EvaGreen ® 是一种用于实时定量 PCR(qPCR)的 DNA 结合染料。该染料的诸多优点使它远胜于 SYBR Green I。除了有相似的光谱特性,EvaGreen 有三个主要特点使它区别于 SYBR Green I。 首先,EvaGreen 对 PCR 的抑制性远 ...
The heart of RT-qPCR is polymerase chain reaction (PCR), a well-established molecular technique that allows researchers to make millions of copies of a single DNA or RNA molecule. Traditional PCR ...
Among the methods of detecting residual DNA, qPCR is the most widely used for residual DNA quantitation due to its sensitivity, accuracy, precision, and time-saving capability. In this webinar, we ...
Scientists use a portable eDNA test to find the world's rarest turtle. This new technology helps conservationists locate ...
BHQnova ™ Probes are double-quenched hydrolysis probes with enhanced quenching and greater signal-to-noise ratio for long probes (>25 bases). Such probes consist of a 5' fluorophore dye, a 3 ...
TaqPath qPCR Master Mix, CG for DNA detection TaqPath qPCR Master Mix, CG from Applied Biosystems is a 2× formulation for gene expression and miRNA analysis that includes a thermostable fast DNA ...
Both the DNA and RNA systems are also suitable for downstream applications such as qPCR or sequencing. For DNA analysis, the EMBER™ Ultra DNA Gel Kit offers the highest sensitivity and resolution ...