Change the medium 1 h before transfection. 2| To prepare the calcium phosphate–DNA coprecipitate, combine 100 μl of 2.5 M CaCl 2 with 25 μg of plasmid DNA in a sterile 5-ml plastic tube and ...

Developed in the late 1980s, liposomal transfection reagents work by enveloping the DNA or RNA and then fusing with the cell membrane to deposit the nucleic acid cargo inside. Roche Applied ...

The most recent and sophisticated multi-component transfection reagent, FuGENE ® 4K, was created to transfer DNA into both difficult and routine mammalian cell lines. Users are able to obtain ...