We recommend nebulizing the DNA for a minimum of 2 min and then analyzing a 5-μl sample of the DNA by gel electrophoresis ... and purify the DNA using QIAquick Gel Extraction kit according ...

Add the powder to 30 ml of extraction ... DNA control' in Step 27. Set aside 1–2 μl from each sample to compare with the aliquot from Step 15; assess the sonication efficiency by gel ...