Nature11 个月
Detecting protein-protein interactions with GFP-fragment reassembly
To prepare the cloning vectors, transform separately pET11a-link-NGFP and pMRBAD-link-CGFP into an E. coli strain that gives high-quality plasmid DNA (for example, DH10B or XL1-Blue) and plate ...
The Scientist1 年
Pain-free Cloning?
Imagine cloning a gene into several different expression vectors without restriction enzymes or ligations. Invitrogen's Echo Cloning System promises just that. Based on the Univector Plasmid-fusion ...