Engineering the replication of target DNA through cloning, or changing its genetic code through mutations, are detail-oriented processes whose foibles can spell disaster. Caitlin Smith looks at ...

Complete the design of the template oligonucleotide by incorporating miR-30 microRNA (miRNA) sequences (as DNA) into the target ... at 0.5 μmol scale from, for example, Sigma-Genosys.

Researchers can quickly and efficiently move between expression systems without subcloning by swapping the insert from vector to vector. Cloning DNA fragments using restriction enzymes is like flying ...