Keep the staining reactions at as low a volume as possible to conserve reagents. For instance, you can add 25ml of a 2´ stock of stain to 25 ml of a 2´ stock of cells for a total volume of 50ml. All ...

We generated a new type of MHC multimers, which can be monomerized in the presence of a competitor, resulting in rapid loss of the staining reagent. Following dissociation, the T cells are ...

We recommend a Wright stain (right) for mouse blood cells rather than Diff-Quick® (left), as the latter doesn't stain mouse eosinophils well. (For each stain, neutrophils are on the left, eosinophils ...

Historically, cell lineage tracing studies have taken large pools of cells from mature tissues and inferred their origins retrospectively by staining the cells with vital dyes or transplanting ...

When more favorable conditions arise the endospore germinates, again forming a viable vegetative cell. The presence of endospores in a bacterial culture can be detected by staining with malachite ...