CRISPR-Cas9 genome editing exploits the CRISPR-Cas ... Here, the authors have designed and optimized circular guide RNA (cgRNA) for Cas12f, which significantly improves the efficiency of gene ...

Editing RNA isn’t all that different from DNA base-editing techniques, which typically use Cas9 or other enzymes attached to a CRISPR guide RNA to replace one nucleotide with another. In the case of ...

Cas9 associates with a guide RNA (an RNA molecule that brings Cas9 to a cleavage site in the DNA target) and then cleaves the target DNA. However, the molecular mechanism of the RNA-guided DNA ...

The team first focused on a structural feature of the CRISPR Cas9 protein that is key to its success as a genome-editing tool; this structural feature is responsible for binding to the enzyme’s RNA ...

In their new work, to find novel programmable systems, the team began by zeroing in on a structural feature of the CRISPR Cas9 protein that binds to the enzyme's RNA guide. That is a key feature ...