Change the medium 1 h before transfection. 2| To prepare the calcium phosphate–DNA coprecipitate, combine 100 μl of 2.5 M CaCl 2 with 25 μg of plasmid DNA in a sterile 5-ml plastic tube and ...

Electroporation works well with cell lines that are refractive to other techniques, such as calcium phosphate–DNA coprecipitation. But as with other transfection methods, the optimal conditions ...

Each of these techniques has its drawbacks: for example, calcium phosphate- mediated transfections suffer ... has introduced the Chariot transfection reagent, which is capable of introducing molecules ...

The human embryonic kidney cell line HEK293 (American Type Culture Collection, Rockville, MD) was transfected using the FuGene transfection ... lysed in 1× potassium phosphate buffer, pH 7.8 ...