Change the medium 1 h before transfection. 2| To prepare the calcium phosphate–DNA coprecipitate, combine 100 μl of 2.5 M CaCl 2 with 25 μg of plasmid DNA in a sterile 5-ml plastic tube and ...

Calcium phosphate–mediated transfection also proved particularly well-suited to the generation of stable transfectants, a feature that enhanced the appeal of this approach to other researchers ...