Gene-editing tools such as CRISPR/Cas9 can be used to create isogenic cell lines, which can be further used to model a specific patient population. An isogenic cell line was created to model ...

We provide transgene expression in mammalian cell lines using lentiviral and retroviral transduction and gene knockout and knockin using CRISPR/Cas9 technologies. 1. STABLE CELL LINE GENERATION ...

Horizon has created a library of ready-made isogenic human cell line pairs edited using CRISPR-Cas9 technology. With 10,000 gene targets available — 3,000 of them off-the-shelf — the knockout ...

The second tool is the CRISPR-Cas9 system, which allows easy and precise editing of any region of the genome. When it comes to traditional immortalized cell lines, such as HeLa or HEK293, cutting with ...

When the CRISPR Cas9 protein is added to a cell along with a piece of guide RNA, the Cas9 protein hooks up with the guide RNA and then moves along the strands of DNA until it finds and binds to a ...

Advances in the gene-editing technology known as CRISPR-Cas9 over the past 15 years have yielded important new insights into ... “We have created four new Cas12a mouse lines that will allow ...

To investigate the regulatory regions controlling gene activation, the team inserted a green fluorescent protein marker into the SNRPN gene, creating cell lines ... CRISPR-associated protein 9 ...