Gene-editing tools such as CRISPR/Cas9 can be used to create isogenic cell lines, which can be further used to model a specific patient population. An isogenic cell line was created to model ...

To illustrate the feasibility of targeted sequencing in conjunction with CRISPR screens ... Human RKO cells harboring Cas9 were transduced with the sgRNA library and, nine days after transduction ...

Mammalian cell gene editing has extensively used RNA-guided CRISPR-Cas systems, which have shown great promise in the treatment of genetic disorders. Class II Cas9 ... system to screen 13 Cas12b ...