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生物通
1 个月
华中农业大学开发出CRISPR/Cas13基因编辑新工具
南湖新闻网讯(通讯员 余博)近日,华中农业大学植物科学技术学院棉花遗传改良团队金双侠教授在Genome Biology在线发表了题为“Systemic evaluation of various CRISPR/Cas13 orthologs for knockdown of targeted transcripts in plants”的研究论文 南湖新闻网讯(通讯员 余博)近日,华中 ...
生物通
1 个月
学院刘亮教授团队发现CRISPR-Cas系统具备反式切割pre-crRNA活性并开发 ...
此外,团队成员也在其他V型CRISPR-Cas系统成员,如Cas12b、Cas12i和Cas12j,以及II型CRISPR-Cas9中观察到了这一现象。与Cas12a稍不同,Cas9仅在单链靶标DNA存在的情况下才会启动对pre-crRNA的spacer区的反式切割,且Cas9的HNH和RuvC结构域对于pre-crRNA的反式切割都是必需的。
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