Over the past 25 years there have been major technological advances in the ways that CD4 + T cells are enumerated, with flow cytometry now generally regarded as the predicate technique.

Cell proliferation may be assessed by flow cytometry. Cell are labelled with the dye CFDA ... The effect of lymphopenia upon antigen-driven proliferation of auto-reactive CD4 T-cells (or CD4 +ve ...

The experimental design of a multicolour flow cytometry experiment has a number of issues that should be carefully ... An example of Level One choice of fluorochromes would be CD3-FITC, CD4-APC and ...