Neither variant was present in the starting BAC DNA. This modified application of direct selection results in the production of clones in a sequence-ready format (in plasmid vectors with inserts ...

Next, individual BAC clones selected for DNA sequence analysis were further fragmented, and the smaller genomic DNA fragments were subcloned into vectors to generate a BAC-derived shotgun library.

First submissions are due Nov. 15. Written requests will be ranked by a peer review committee based on such criteria as the importance of the organism, uses of the BAC library other than for genomic ...