作为Western Blot（WB ... Rabbit mAb #12556和 β-Actin (D6A8) Rabbit mAb #8457， 对Saos-2和LNCaP细胞的提取物进行蛋白质印迹分析。 b. 翻译后修饰蛋白的阳性对照 ...

作者首先检索了已发表的芯片数据和质谱数据，发现常用的细胞骨架蛋白（actin，actinin和各种tubulin异构体）、线粒 ... 脾和脂肪组织（性腺）。比例尺=1厘米。B：Odyssey定量Western blot结果展示β-肌动蛋白（绿色）的表达水平在肌肉，心脏，骨骼，颅骨，脾和脂肪 ...

期刊杂志对Western Blot文章发表的要求越来越严，这些要求包括如何确保Western Blot的重复性、定量的准确性、均一化方法的选择等。Nature、JCB、JBC等杂志明确规定Western Blot进行均一化时，要求选择不受实验条件影响的方法；JBC甚至还建议优先选择总蛋白进行均一化。

Beta-actin is commonly used as a western blot loading control as is expressed within all eukaryotic cell types and is not affected by cellular treatments. Beta-actin is approximately 42 kDa and ...

Western bolting is a well-established method that is currently relied on in biology laboratories for determining the specific proteins and their abundance within a given sample of cells or tissue.

94, 11669-74 (1997). 3 Liu NK, Xu XM. Beta-tubulin is a more suitable internal control than beta-actin in western blot analysis of spinal cord tissues after traumatic injury. J Neurotrauma.

This application note compares the linearity of a series of dilutions measured by stain-free total protein measurement as well as immunodetection of three housekeeping proteins (ß-actin, ß-tubulin, ...