Beta-actin is commonly used as a western blot loading control as is expressed within all eukaryotic cell types and is not affected by cellular treatments. Beta-actin is approximately 42 kDa and ...

Western bolting is a well-established method that is currently relied on in biology laboratories for determining the specific proteins and their abundance within a given sample of cells or tissue.

Western blotting ... Western blotting experiments and enables loading control calculations over a wide protein-loading range. HKP-based normalization with GAPDH, actin, or tubulin needs to be ...

94, 11669-74 (1997). 3 Liu NK, Xu XM. Beta-tubulin is a more suitable internal control than beta-actin in western blot analysis of spinal cord tissues after traumatic injury. J Neurotrauma.

Here we found that in resting lymphocytes, centrosome-associated Arp2/3 locally nucleates F-actin, which is needed ... Silencing efficiency was analysed by western blot as described below.

This application note compares the linearity of a series of dilutions measured by stain-free total protein measurement as well as immunodetection of three housekeeping proteins (ß-actin, ß-tubulin, ...