PCR involves the enzymatic amplification of nucleic acid templates, and can be divided into four major steps, listed below. 1 Denaturation: Double-stranded DNA separates into single strands. Annealing ...

which melts double-stranded DNA into single strands; annealing, which lets small pieces of primer DNA bind to either side of the region of interest; and elongation, where the enzyme DNA polymerase ...

Because DNA's makeup is determined by a simple set ... This generative design process called shape annealing was pioneered by ...