在Western Blot实验中，用来进行均一化的内参是否合适，是否可以作为绝对定量的均一化的标准？是需要研究者首先在心中打个问号的。那么如何确定选择的内参是否合适？——LI-COR 提供的 Odyssey® Loading Indicators（OLI）可以帮助您解决这个问题。

作为Western Blot（WB）的新手或老手，可能已经对WB实验要点了然于胸，例如，须要使用新鲜样本、根据蛋白分子量选择合适孔径的转印膜、根据抗体 ...

期刊杂志对Western Blot文章发表的要求越来越严，这些要求包括如何确保Western Blot的重复性、定量的准确性、均一化方法的选择等。Nature、JCB、JBC等杂志明确规定Western Blot进行均一化时，要求选择不受实验条件影响的方法；JBC甚至还建议优先选择总蛋白进行均一化。

Beta-actin is commonly used as a western blot loading control as is expressed within all eukaryotic cell types and is not affected by cellular treatments. Beta-actin is approximately 42 kDa and ...

Western blotting frequently requires optimization of blocking reagents, ... HKP-based normalization with GAPDH, actin, or tubulin needs to be optimized for antibody dilutions, ...

As well as regular behavioral testing to determine pain sensitivity, protein samples were taken from the mice and run in a western blot. Blots were incubated with antibodies against ROR2, ...

Important considerations for protein analyses using antibody-based techniques: down-sizing Western blotting up-sizes outcomes. The Journal of Physiology , 591(23), pp.5823-5831.

Bio-Rad Laboratories Inc. has launched StarBright™ Blue 700, a fluorescent dye labeled secondary antibody for use in multiplex western blotting with a two- to fourfold lower limit of detection than ...