AMPKα knockout cells exhibit suboptimal autophagy induction in response ... and to the rest proteins at 1:1,000 dilutions for western blot. AMPK immunoprecipitation was performed using 1 ...

研究发现AMPK信号通过磷酸化Beclin1的苏氨酸388位点从而调控细胞自噬，进一步揭示了自噬的发生发展的分子机制，为解析细胞自噬奠定了一定的理论基础。 自噬领域国际学术期刊Autophagy 在线发表了中国科学院上海生命科学研究院营养科学研究所刘知学组的研究 ...

为解决周围神经损伤(PNI)临床治疗难题，研究人员探讨了天然黄酮类化合物柚皮苷(naringin)对血清饥饿诱导的雪旺细胞(SCs)凋亡 ...

创伤性脑损伤是一种后天获得性损伤形式，由外部物理冲击头部而导致脑损伤。 皮肤癌是美国最常见的癌症，每年约有500万例。黑色素瘤与其他类型 ...

2018年3月12日 讯 /生物谷BIOON/ --当白色脂肪组织中积累的能量发生过剩就会引起肥胖，而棕色脂肪组织可以通过产热过程对能量进行解偶联从而发挥 ...

AMPK, a highly conserved sensor of cellular energy status, is found in all eukaryotic cells and maintains metabolic homeostasis by reprogramming growth, metabolism, and autophagy in the face of ...

In addition, a low-energy status activates ULK1/2 through AMP-activated protein kinase (AMPK). The second signal class involves autophagy cargoes, such as damaged mitochondria, which can also ...

Akt may also negatively regulate autophagy by phosphorylating Beclin 1. AMP-activated protein kinase (AMPK), which is regulated by AMP levels, negatively regulates mTOR and also directly ...

Conversely, autophagy is inhibited by mTOR, a sensor of nutrient availability and recipient of growth factor signaling. AMPK activation may interfere with mTOR-mediated inactivation of ULK1.

The researchers also identified a decline in autophagy-regulating pathways, such as mTORC1 hyperactivation and reduced AMPK activity, with age. In Alzheimer's disease, autophagy impairment was ...

"A really interesting finding was when Matt (lead author of the study, Matthew Ulgherait) activated AMPK in the nervous system, he saw evidence of increased levels of autophagy in not only the ...