To track the expression of a specific AAV genome in a pool, researchers add a unique pair of small barcodes consisting of a few nucleotides to the AAV sequence. Each viral genome also contains two ...

To track the expression of a specific AAV genome in a pool, researchers add a unique pair of small barcodes consisting of a few nucleotides to the AAV sequence. Each viral genome also contains two ...

ddPCR technology then amplifies and detects the ligated oligo pairs, while an ITR-2 assay simultaneously detects the AAV genome. Software analyzes the signals from each capsid type to calculate ...

Gator Bio is the market’s sole solutions supplier that provides assays for genome titer and integrity, capsid titer, and empty-full ratio on a single automated platform. The AAV genome titer and ...

Single AAV Systems for HDR-Mediated Genome Editing in Vivo Dual AAV Systems for HDR-Mediated Genome Editing in Vivo ...

Maple syrup urine disease (MSUD) is a rare genetic inborn error of metabolism characterized by recurrent life-threatening neurologic crises and progressive brain ...

As such, AAV-mediated gene editing is highly efficient (up to 20 percent targeting efficiency ... In addition, mutations in cohesin are increasingly seen in cancer genome sequencing. These and other ...

Next-generation sequencing (NGS) is a promising technology for sequence confirmation of viral genome and detection ... quality control methods for AAV-based viral vectors. The outcome of this ...

To overcome these barriers, researchers explored lentiviral vectors, which integrate into the genome and bypass AAV-specific limitations. In the study, "Lentiviral Gene Therapy with CD34 ...